Low clustering possible causes (1200 to 1400 K/mm2 expected for Illumina MiSeq v3):
– Imprecise quantification of final libraries
– Inaccurate dilution/mixing of libraries before loading
– Denaturation issues
– Insufficient mixing of the MiSeq reagent cartridge (normally requires 10x inversion once thawed)
High clustering possible causes:
– Imprecise quantification of final libraries
– Inaccurate dilution/mixing of libraries before loading on the MiSeq