The Polyphen-2 prediction scores automatically displayed in AlamutTM Visual Plus are extracted from the WHESS database (http://genetics.bwh.harvard.edu/pph2/dbsearch.shtml). The WHESS database contains a pre-computed set of PolyPhen-2 predictions for the Whole Human Exome Sequence Space. 
Whereas, the scores obtained on the site using the batch query (http://genetics.bwh.harvard.edu/pph2/bgi.shtml) are generated upon each request. 

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