What are the criteria for a region to be classified as “off target” in the low confidence variants?

Variants outside the target region defined by the solution are filtered as “off-target”.

– For capture based enrichment, many off-target variants may be observed in regions flanking the target region that still have sufficient coverage for variant calling.

– For amplicon-based enrichment, if the variant is located in the region covered by a primer and no other amplicons overlap this region, they are classified as “off target”.