Usually with most of our panels the amount of DNA we quantify after the capture is between 10 and 30 ng/µl. Low yields may be related to several problems:

  • incomplete denaturation step 
  • a decrease of temperature after denaturation and prior to the addition of the probes
  • omission of probe addition
  • Loss of material during the wash steps (?)
  • Problems with post-capture amplification
  • Loss of material during cleanup