Is it possible to perform captures with only 1 sample when using a Bundle Solution by SOPHiA GENETICS?

No. It is not possible to perform captures with only 1 sample.

The SOPHiA capture protocols have been optimized to work in general with 4, 8 and 12 samples per capture*.

– These formats are designed to optimize multiplexing for each solution.

– Performing captures with only 1 sample would require a higher amount of starting DNA to obtain good results.

– Efficient captures require a minimum amount of DNA (between 1.2 µg and 1.8 µg for our protocols) and an appropriate number of probes.

* The Whole Exome Solution by SOPHiA GENETICS is the only kit where a capture with 3 samples is possible due to the large amount of probes.