How long can individual libraries and captured libraries be stored at -20°C before sequencing?

A

How long can individual libraries and captured libraries be stored at -20°C before sequencing?

When stored in low bind tubes, DNA being very stable, libraries may stay at –20°C for months/years prior to usage.

After a long-term storage, it is preferable to repeat quantification by a fluorometric quantitation (e.g. Qubit®, Thermo Fisher Scientific) before usage.